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Minimum inhibitory concentration. In microbiology, the minimum inhibitory concentration ( MIC) is the lowest concentration of a chemical, usually a drug, which prevents visible in vitro growth of bacteria or fungi. [1] [2] MIC testing is performed in both diagnostic [1] [2] and drug discovery laboratories.
Thin paper discs containing an antibiotic have been placed on an agar plate growing bacteria. Bacteria are not able to grow around antibiotics to which they are sensitive. This is called "the zone of inhibition". Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics.
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent. The MBC is identified by determining the lowest ...
Etest is a pre-prepared non-porous plastic reagent strip with a predefined gradient of antibiotic, covering a continuous concentration range. It is applied to the surface of an agar plate inoculated with the test strain, where there is release of the antimicrobial gradient from the plastic carrier to the agar to form a stable and continuous ...
The broth microdilution method can be used to test the susceptibility of microorganisms to multiple antibiotics at once. [3] Broth microdilution is also highly accurate. The accuracy of its results are comparable to agar dilution, the gold standard of susceptibility testing. Other advantages include the commercial availability of plates, the ...
The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture -based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the ...
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria. [1] : 149 [2]
Harvard sentences. The Harvard sentences, or Harvard lines, [1] is a collection of 720 sample phrases, divided into lists of 10, used for standardized testing of Voice over IP, cellular, and other telephone systems. They are phonetically balanced sentences that use specific phonemes at the same frequency they appear in English.